While queens have tested positive for AFB spores, it is unlikely that they would carry enough spores to create an infection. Where spore-carrying queens from AFB infected hives were intentionally placed in uninfected hives, they did not infect the new colonies.
Package bees are a broodless nucleus colony comprised of a caged queen and an artificial swarm of bees. Package bees can be shipped long distances, and then installed into hives with empty comb in order to start new honey bee colonies. Although package bees contain no brood, they have been shown to be capable of carrying sufficient spores to produce an AFB infection once the bees begin producing brood in the new hive.
Queens are unlikely to transmit AFB but package bees can.